Comparison of field inversion gel electrophoresis with contour-clamped homogeneous electric field electrophoresis as a typing method for Enterococcus faecium

Validity of contour-clamped homogeneous electric field electrophoresis as a typing system for Candida albicans.

Instigated by an increase in serious human Candida infections and aided by advances in technology, there has been renewed interest in the study of the epidemiology of fungal infections. Among the newer techniques available, contour-clamped homogeneous electric field (CHEF) electrophoresis has shown great promise as a tool for typing strains of Candida albicans. However, few studies have address...

Typing of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis.

Polymerase chain reaction (PCR) with JB1 or REP consensus oligonucleotides and pulsed field gel electrophoresis (PFGE) were used to study genomic DNA extracted from 31 strains of enterococci. Eleven ATCC strains, representative of 11 species of Enterococcus, were initially tested by JB1-PCR, revealing that Enterococcus malodoratus and Enterococcus hirae presented identical banding patterns. Eig...

The Use of Pulsed Field Gel Electrophoresis in Listeria monocytogenes Sub-Typing – Comparison with MLVA Method Coupled with Gel Electrophoresis

Field inversion gel electrophoresis as a typing system for group A streptococcus.

Field inversion gel electrophoresis (FIGE) was performed on 139 isolates of group A streptococcus (GAS) representing 72 different M types. Multiple isolates were tested for 27 M types (range, 2-19). Each of the M types tested had at least one unique banding pattern that was not observed in any other M type. For 23 of 27 M types for which multiple isolates were available, band profiles of the is...

Pulsed homogeneous orthogonal field gel electrophoresis (PHOGE).

A versatile system (PHOGE) has been developed that allows resolution of molecules of DNA megabase pair size by the use of homogeneous, orthogonal, pulsed fields. The resulting electrophoretograms have characteristics that differ from those produced by other systems for pulsed field electrophoresis. Molecules in a two-fold range of sizes can be separated with maximum resolution, or a much larger...